Skip to main content
. 2015 Jul 10;112(30):9334–9339. doi: 10.1073/pnas.1503653112

Fig. 6.

Fig. 6.

Loss of Rnaseh1 leads to the retention of RNA patches on the H- and L-strands close to LSP, some of which are contiguous with DNA at both ends. BclI-digested mtDNA from MEFs cultured for 9 d without (control) or with (ΔRH1) 4HT was fractionated by (A) 2D-AGE or (B–D) 1D-AGE and blot hybridized to the indicated probes. For 1D-AGE, samples were denatured before loading. (Inset below A) Blocked BclI site at np 16,179 predicts a fragment of 6.6 kb. The BclI product of ∼6.6. kb was gel extracted, denatured, and refractionated after treatment without or with Eco-RNase HI (RH) or (D)Nase (B–D); next to the gel images are interpretations of the single-stranded products and (E) the possible double-stranded combinations, with Ori-H and Ori-b conflated to Ori. (B) Gel-extracted 4.15-kb fragment (1n) provided an additional marker, and in D, the same 1n material enabled comparison of the relative amounts of Ori-H and Ori-b ends of the 1n and 6.6-kb fragments.