Fig. S4.

αO-contoxins GeXIVA[1,2] and GeXIVA[1,4] do not affect voltage-dependent calcium current in rat DRG neurons. Different DRG neurons were exposed for 2.5 min to 1 μM of either GeXIVA[1,2] (A) or GeXIVA[1,4] (C). Currents were elicited at the indicated voltages (Materials and Methods) once every 10 s, and are shown before (gray trace), during (black trace), and upon (gray trace) recovery from toxin. The charge carrier was 5 mM Ba²⁺. (B and D) Time course is shown for the data depicted above. The asterisks indicate the times when the currents shown above were generated. The solid bar indicates the duration of toxin application. (E) Average effect of toxin on voltage-dependent calcium current (n = 8, 8). The duration of toxin application ranged from 1.2 to 3.1 min for GeXIVA[1,2] (mean = 1.7 min) and from 1.5 to 2.5 min for GeXIVA[1,4] (mean = 1.9 min). Neither toxin significantly affected calcium current (P > 0.05). The neuronal diameter ranged from 21 to 34 μm (mean = 27 μm) for the GeXIVA[1,2]-exposed cells and from 26 to 32 μm (mean = 30 μm) for the GeXIVA[1,4]-exposed cells.