Table S1.
Protein precursor sequences of αO-related Conus gene superfamilies
| Superfamily | Peptide | Cys framework | Precursor sequence (ER signal peptide, N-terminal proregions, ↓mature peptide, and ↓C-terminal cleavage site) | Source |
| O1 | αO-GeXIVA | C-C-C-C | MKLTCVLIITVLFLTACQLTTAVTYSRGEHKHRALMSTGTNYRLPK↓TCRSSGRYCRSPYDRRRRYCRRITDACV* | This study |
| MVIIA | C-C-CC-C-C | MKLTCVVIVAVLLLTACQLITADDSRGTQKHRALRSTTKLSTSTR↓CKGKGAKCSRLMYDCCTGSCRSGKC↓G | (86) | |
| O2 | BeB54 | C-C-CC-C-C | MEKLTILLLVAAVLMSTQALIQSDGEKRQQAKINFLS.RK↓STAESWWEGECKGWSVYCSWDWECCSGECTRYYCELW | (87) |
| O3 | CaFr179 | C-C-CC-C-C | MSGLGIMVLTLLLLVFMEASHQDAGEKQATQRDAINVRRRRSLARR↓TVTEECEEDCEDEEKHCCNTNNGPSCARLCF↓G | (87) |
| J | PIXIVA | C-C-C-C | MPSVRSVTCCCLLWMMFSVQLVTPGSPGTAQLSGHRTAR↓FPRPRICNLACRAGIGHKYPFCHCR↓GKRDAVSSSMAV | (76) |
| L | LtXIVA | C-C-C-C | MKLSVMFIVFLMLTMPMTCAGISRSATNGGEADVRAHDKAANLMALLQER↓MCPPLCKPSCTNC↓G | (78) |
| A | α-BuIA | CC-C-C | MFTVFLLVVLTTTVVSFPSDRASDGRNAAANDKASDVVTLVLK↓GCCSTPPCAVLYC↓GRRR | (88) |
| αA-PIVA | CC-C-C-C-C | MFTVFLLVVLATTVVSFTSDRASDDRNTNDKASRLLSHVVR↓GCCGSYPNAACHPCSCKDRPSYCGQ↓GR | (89) | |
| B1 | Conantokin-Br | No Cys | MQLYTYLYLLVPLVTFHLILGTGTLGHGGALTERRSTDATALK | (72) |
| PEPVLQKSAARSTDDNGKDRLTQMKRILKKRGKNAR↓GDEEYSKFIEREREAGRLDLSKFP | ||||
| B2 | Mr8.2 | C-C-C-C-C-C-C-C-C-C | MLRLITAAVLVSACLAYPQKKRTPPQTRPTSR↓ALVSQCRPCPTCRECKCRECKCRECQCRIHSCLSAWDSRGIWMRT | (73) |
| B3 | αB-VxXXIVA | C-CC-C | METLTLLWRASSSCLLVVLSHSLLRLLG↓VRCLEKSGAQPNKLFRPPCCQKGPSFARHSRCVYYTQSRE | (71) |
| C | αC-PrXA | C-C | MQTAYWVMVMMMVMWITAPLSEGGKPKLIIRGLVPNDLTPQRILRSLISGR↓TYGIYDAKPPFSCAGLRGGCVLPPNLRPKFKE↓GR | (83) |
| D | αD-VxXXA | C-CC-C-CC-C-C-C-C | MPKLEMMLLVLLIFPLSYFIAAGGQVVQVDRRGDGLAGYLQRGDR↓DVQDCQVSTPGSKWGRCCLNRVCGPMCCPASHCYCVYHRGRGHGCSC | (90) |
| S | αS-RVIIIA | C-C-C-C-C-C-C-C-C-C | MMSKMGAMFVLLLLFTLASSQQEGDVQARKTHPKREFQRILLRSGR↓KCNFDKCKGTGVYNCGESCSCEGLHSCRCTYNIGSMKSGCACICTYY | (86) |
Sequences and domain definitions are provided in ConoServer (91).
*
DNA sequence of GeXIVA precursor with GenBank accession no. KP793740 is as follows: ATGAAACTGACGTGCGTGTTGATCATCACCGTGCTGTTCCTGACGGCCTGTCAACTCACTACAGCTGTGACTTACTCCAGAGGTGAGCATAAGCATCGTGCTCTGATGTCAACTGGCACAAACTACAGGTTGCCCAAGACGTGCCGTAGTTCCGGTCGTTATTGTCGCTCACCTTATGATCGCCGCAGAAGATATTGCAGACGCATTACGGATGCGTGCGTATAG.