Fig. 1.
ics, Encoding Rsu1, is required for normal ethanol responses. Here, flies were exposed to a 130:20 ethanol:airflow rate, and bars represent means ± SEM. ST50 stands for the median sedation time; increased ST50 indicates reduced ethanol sensitivity. (A) Mutant icsG4 flies show reduced sensitivity to ethanol-induced sedation. This phenotype and (Inset) the loss of Rsu1 protein are rescued with expression of Rsu1 cDNA (UAS-Rsu1; transgene presence indicated by √; n = 8). C, control; M, mutant; R, rescue. ***P < 0.001. (B) Brain expression pattern of icsG4 revealed by a membrane-bound GFP reporter (UAS-mCD8-GFP; green). B shows (Upper) anterior (ant.) and (Lower) posterior (post.) confocal stacks of icsG4 (Left) heterozygous WT and (Right) homozygous mutant flies. Expression includes neurosecretory cells in the pars intercerebralis (PI) as well as the MBs. Neuropil is counterstained with anti-Brp nc82 antibody (red).