Fig. 5.

Alcohol consumption preference phenotypes in flies lacking Rsu1. (A) ics Mutant flies show increased naïve ethanol preference compared with control in the two-bottle choice CAFÉ assay. This phenotype is rescued by expression of UAS-Rsu1 in all icsG4-expressing cells. (B and D) ics Rescue flies lacking Rsu1 expression in the MBs only (ics^G4 UAS-Rsu1 MB-Gal80) do not develop acquired ethanol preference but (B) have normal naïve preference on day 1 and (D) ethanol-induced sedation. (C) Adult MB-specific knockdown of Rsu1 or overexpression of Rac1^CA causes loss of acquired ethanol preference. The transgenes were expressed in adults using a mifepristone-inducible MB-GeneSwitch driver. ns, Not significant; ST-50, median sedation time. *P < 0.05; **P < 0.01 (ics^G4 vs. control); ***P < 0.001.