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Affinity maturation mutations in H5.3 do not contact H5. There are 11 mutations in H5.3, shown in stick representation (4 in the heavy chain and 7 in the light chain), compared with its UCA, none of which are involved in the H5.3-H5 interface. H5.3 light chain is in purple, heavy chain is in teal, residues mutated from the H5.3 UCA are shown as blue sticks, H5hd is in gold, and H5 residues involved in the interface are shown as gray sticks.
