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N7-MTase activity of nsp14 and its mutants. Nsp14 and its mutants were tested for their ability to methylate 32P-labeled G*pppA-RNA. After incubation for 6 min at 37 °C, the reaction products were digested by nuclease P1 into single nucleotides and methylated m7G*pppA or unmethylated G*pppA. They were analyzed by separating the mixture using TLC. The position of origin is marked on the left. The percentage of conversion of G*pppA-RNA to m7G*pppA-RNA is listed at the bottom.
