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. 2015 Jul 13;112(30):9418–9423. doi: 10.1073/pnas.1507087112

Fig. 1.

Fig. 1.

Anti-NCL scFV 4LB5 specifically binds NCL in vitro and on the cell surface. (A) 4LB5 affinity for recombinant NCL was assessed by ELISA using different amounts of scFv. Apparent Kd is also indicated. Curve equation and R2 are also reported. (B) ELISA performed on MDA-MB-231 using different amounts of 4LB5. Curve equation and R2 are also reported. (C) ELISA performed using different amounts of 4LB5 on MDA-MB-231 cells following control (siCTRL) or anti-NCL (siNCL) siRNA transfection. *P < 0.05, **P < 0.01. (D) ELISA on MCF-10a (surface-NCL−) and MDA-MB-231 (surface-NCL+) breast cancer cells. **P < 0.01. All of the experiments are representative of three independent experiments performed in triplicate. Mean ± SD is reported.