4LB5 binds NCL on the surface of different cancer cell lines. Indicated cell lines—(A) MCF-10a normal-like breast, (B) MDA-MB-436 basal B TNBC, (C) BT-549 basal B TNBC, (D) Huh7 HCC, (E) MDA-MB-231 basal B TNBC, (F) T47D luminal breast cancer, (G) PLC-PRF, HCC—were stained (red line) or not (light blue line) for 1 h with 2 μg/mL Cy5.5-labeled 4LB5 and analyzed by flow cytometry. Mean fluorescent index is also reported in parenthesis. Data are representative of three independent experiments performed in duplicate.