Fig. S4.

Normal surface localization and LGI1 binding of ADAM22dC4 mutant. FAH-tagged ADAM22 (A and B) and His_x6-tagged LGI1 were expressed in dissociated hippocampal neurons. (C) Surface-bound LGI1 (red), surface-expressed ADAM22 (green), and total ADAM22 (blue) were labeled by anti-His_x6, FAH, and HA antibodies, respectively. Wild-type ADAM22 is surface localized and binds extracellular LGI1. ADAM22 lacking the PDZ-binding motif (ADAM22dC4, Bottom) is also surface localized and able to bind extracellular LGI1. (Scale bar, 10 μm.)