Skip to main content
. 2015 Jul 9;112(30):E4055–E4064. doi: 10.1073/pnas.1501967112

Fig. 3.

Fig. 3.

MYC expression regulates expression of downstream transcription factors (POU3F2, SOX2, and OLIG2) that mediate tumorigenicity. (A) Silencing of MYC by independent shRNAs suppressed expression of POU3F2, SOX2, and OLIG2 (qRT-PCR). Error bars, SD. (B) Exogenous MYC expression in U87MG induced expression of POU3F2, SOX2, and OLIG2 (qRT-PCR). Error bars, SD. (C) Silencing of POU3F2, SOX2, and OLIG2 by independent siRNAs (Top) did not affect MYC expression (Bottom, qRT-PCR). Error bars, SD. (D) Increased expression of MYC, POU3F2, SOX2, and OLIG2 in a tumorigenic Cdkn2a (Ink4a/Arf), Pdgfb+ glioblastoma line relative to its nontumorigenic precursor Cdkn2a (Ink4a/Arf) astrocyte line (qRT-PCR). Error bars, SD. (E) IHC staining for MYC and OLIG2 in a panel of 65 human glioblastoma specimens. (Left) Representative staining in one specimen. (Right) Percentages of specimens staining positive versus negative for OLIG2 grouped by MYC staining. (F) Colocalization by IF of MYC and OLIG2 in a representative human glioblastoma specimen, with MYC in green, OLIG2 in red, and colocalization in yellow (marked by arrowheads).