Fig. S2.

TS and xenograft formation assays in short-term–passaged glioblastoma lines recapitulate findings in the established glioblastoma line U87MG. (A) Subclones of the glioblastoma line 83 were passaged under serum/adherent conditions and tested for ability of single-cell suspensions to propagate as TSs. In parallel, the subclones were tested for ability to form s.c. xenografts. A plus sign denotes formation of at least one tumor in the five mice injected with the various subclones. Results of two independent experiments are shown (Left). Vertical scatter plot depicts frequencies of TS formation grouped by xenograft formation ability per Exp 1 (Right). (B) As above, but with the primary glioblastoma line CMK3. (C) Comparison of variances in frequencies of TS formation of U87MG subclones, relative to that expected from variation in experimental handling. For the latter, experiments were repeated 10 times using a mixture of 20% GFP-expressing U87MG and 80% non–GFP-expressing U87MG, which approximates the deterministic model (Left). The Levene test for equality of variances was performed, with asterisks denoting statistically different variances (Right).