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. 2015 Jul 9;112(30):E4055–E4064. doi: 10.1073/pnas.1501967112

Fig. S5.

Fig. S5.

MYC expression drives expression of downstream transcription factors that promote glioblastoma stem-like states. (A) Assessment by RT-PCR of relative expression of MYC and transcription factors that mediate reprogramming of glioblastoma into more stem-like states (12), following MYC knockdown by two independent shRNAs in the glioblastoma line 83. (B) Assessment by RT-PCR of relative expression of the reprogramming transcription factors (Top) and MYC (Bottom), following knockdown of each transcription factor by two independent siRNAs in the glioblastoma line 83. Error bars, SD. (C). Assessment by RT-PCR of relative expression of the reprogramming transcription factors and MYC in nontumorigenic Tp53−/− astrocytes compared with tumorigenic Tp53−/−; Pten+/− glioblastoma cells. The tumorigenic glioblastoma line exhibited increased expression of MYC, POU3F2, SOX2, and OLIG2 relative to the nontumorigenic precursor astrocyte line. Error bars, SD.