Figure 4.

Inhibitory effects of YGSHT on triglyeride contents and GPDH activity in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with MDI for 8 days. The cells were exposed to various concentrations of YGSHT during the differentiation period. (a) The triglyceride contents were measured enzymatically using a commercial kit at 570 nm. (b) GPDH activity of the cells was assessed by measuring the decrease in NADH at 340nm using a GPDH activity assay kit. Data are presented the mean ± SEM. ***p < 0.001 compared with the differentiated control