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Inhibitory effects of YGSHT on leptin production in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with MDI for 8 days. Culture supernatant was collected from the YGSHT-treated cells. Leptin production was determined by ELISA by subtracting the value measured at 450 nm using a mouse leptin immunoassay kit (R&D Systems). Data are presented the mean ± SEM. *p < 0.05 and ***p < 0.001 compared with the differentiated control
