Figure 3.

Luteolin inhibited both mRNA and protein expression of inducible nitric oxide synthase (iNOS), and the production of nitric oxide in TLI stimulated 3T3-L1 adipocytes. The cells were pretreated with various concentration of luteolin and then induced with TLI for 6 h for mRNA expression and 24 h to assay protein levels and nitric oxide (NO) production. Effect of luteolin in TLI-induced (a) mRNA and (b) protein expression and (c) band intensity ratio of iNOS and actin and (d) NO production were evaluated. Data are expressed as the mean ± standard error of the mean (n = 3). *P < 0.05. TLI: Tumor necrosis factor-α+ lipopolysaccharide + interferon γ 10 ng/ml, L: Luteolin