Table 6.
The intra- and inter-day precision for BoNT-C spiked in chicken serum after 4 and 24 h of incubation.
| NTP/IS | CTP/IS | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Day | Mean | RSDr (%) | RSDR (%) | x blank | Mean | RSDr (%) | RSDR (%) | x blank | |
| Blank 4 h (24 h) | 1 | 0.23 (0.35) | 13 (17) | - | 0.26 (0.58) | 14 (37) | - | ||
| 2 | 0.24 (0.32) | 9 (15) | 16 (21) | - | 0.29 (0.48) | 8 (16) | 19 (28) | - | |
| 3 | 0.18 (0.32) | 14 (27) | - | 0.22 (0.47) | 23 (15) | - | |||
|
| |||||||||
| 50 MLD50 BoNT-C 4 h (24 h) | 1 | 0.43 (1.1) | 12 (12) | 1.9 (3.1) | 0.78 (2.3) | 9 (11) | 3.0 (4.0) | ||
| 2 | 0.42 (0.71) | 27 (29) | 18 (26) | 1.8 (2.0) | 0.74 (1.1) | 27 (34) | 17 (34) | 2.6 (2.0) | |
| 3 | 0.43 (0.83) | 9 (15) | 2.4 (2.4) | 0.78 (1.5) | 10 (15) | 3.6 (2.7) | |||
|
| |||||||||
| 250 MLD50 BoNT-C 4 h (24 h) | 1 | 1.5 (4.5) | 7 (26) | 6.5 (13) | 3.9 (12) | 10 (28 | 15 (21) | ||
| 2 | 1.5 (3.1) | 11 (13) | 14 (40) | 6.1 (8.7) | 3.8 (6.7) | 4 (14) | 16 (42) | 13 (12) | |
| 3 | 1.3 (2.0) | 20 (23) | 7.4 (5.8) | 3.1 5.4) | 22 (14) | 15 (9.5) | |||
|
| |||||||||
| 500 MLD50 BoNT-C 4 h (24 h) | 1 | 2.4 (5.5) | 15 (15) | 10 (16) | 6.3 (13) | 16 (24) | 24 (24) | ||
| 2 | 2.2 (4.6) | 10 (15) | 15 (19) | 9.2 (13) | 6.4 (12) | 7 (13) | 13 (19) | 22 (21) | |
| 3 | 2.4 (4.0) | 17 (12) | 13 (11) | 6.2 (11) | 14 (7) | 29 (19) | |||
NTP/IS = The ratio between the intensities of the N terminal cleavage product and the internal standard peptide in the same spectrum.
CTP/IS = The ratio between the intensities of the C terminal cleavage product and the internal standard peptide in the same spectrum.
RSDr= the relative standard deviation in percent calculated from 6 replicates, each spotted and analyzed 3 times.
RSDR= the relative standard deviation in percent calculated from 6 replicates, each spotted and analyzed 3 times on 3 different days.
x blank = the mean NTP/IS (or CTP/IS) in a sample (6 replicates, 3 spots each) divided by the mean of the same ratio of the blank samples (6 replicates, 3 spots each).