Table 7.
The intra- and inter-day precision for BoNT-D/C spiked in chicken serum after 4 and 24 h of incubation.
| NTP/IS | CTP/IS | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Day | Mean | RSDr (%) | RSDR (%) | x blank | Mean | RSDr (%) | RSDR (%) | x blank | |
| Blank 4 h (24 h) | 1 | 0.01 (0.02) | 15 (14) | - | 0.02 (0.03) | 10 (12) | - | ||
| 2 | 0.01 (0.02) | 15 (12) | 29 (31) | - | 0.03 (0.04) | 6 (7) | 12 (24) | - | |
| 3 | 0.02 (0.03) | 35 (14) | - | 0.02 (0.03) | 14 (35) | - | |||
|
| |||||||||
| 1 MLD50 BoNT-D/C 4 h (24 h) | 1 | 0.02 (0.06) | 12 (22) | 1.6 (3,8) | 0.04 (0.14) | 16 (23) | 1.6 (4.2) | ||
| 2 | 0.02 (0.09) | 17 (23) | 31 (38) | 1.9 (5,9) | 0.05 (0.22) | 7 (19) | 21 (48) | 1.9 (5.7) | |
| 3 | 0.03 (0.11) | 28 (33) | 2.0 (4,1) | 0.05 (0.10) | 26 (77) | 1.9 (3.7) | |||
|
| |||||||||
| 10 MLD50 BoNT-D/C 4 h (24 h) | 1 | 0.09 (0.41) | 35 (35) | 7.3 (26) | 0.17 (0.99) | 31 (40) | 7.0 (30) | ||
| 2 | 0.11 (0.60) | 11 (24) | 32 (47) | 8.9 (39) | 0.28 (1.8) | 4 (19) | 35 (56) | 10 (46) | |
| 3 | 0.14 (0.64) | 29 (58) | 8.8 (24) | 0.19 (0.53) | 42 (51) | 7.6 (21) | |||
|
| |||||||||
| 20 MLD50 BoNT-D/C 4 h (24 h) | 1 | 0.20 (1.5) | 16 (33) | 16 (92) | 0.42 (2.9) | 23 (27) | 17 (86) | ||
| 2 | 0.20 (1.2) | 13 (30) | 45 (33) | 16 (78) | 0.51 (2.8) | 7 (20) | 38 (55) | 19 (73) | |
| 3 | 0.42 (1.5) | 24 (31) | 26 (54) | 0.27 (0.70) | 64 (43) | 11 (27) | |||
NTP/IS = The ratio between the intensities of the N terminal cleavage product and the internal standard peptide in the same spectrum.
CTP/IS = The ratio between the intensities of the C terminal cleavage product and the internal standard peptide in the same spectrum.
RSDr= the relative standard deviation in percent calculated from 6 replicates, each spotted and analyzed 3 times.
RSDR= the relative standard deviation in percent calculated from 6 replicates, each spotted and analyzed 3 times on 3 different days.
x blank = the mean NTP/IS (or CTP/IS) in a sample (6 replicates, 3 spots each) divided by the mean of the same ratio of the blank samples (6 replicates, 3 spots each).