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. 2014 Dec 23;15(1):1174. doi: 10.1186/1471-2164-15-1174

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© Redshaw et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MethyLight duplex dPCR. (A) Duplex p14 dPCR assay showing data for p14_M and p14_M2 assays separately and with estimated targets for both assays combined. (B) dPCR heatmap showing distribution of p14_M (red) and p14_M2 (blue) positive chambers in a duplex reaction showing three example panels of a dPCR plate. (C) Correlation between MethyLight qPCR vs. duplex dPCR (estimated targets for both assays combined). All correlations were significant at p < 0.0001.