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. 2014 Dec 23;15(1):1174. doi: 10.1186/1471-2164-15-1174

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© Redshaw et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of DNA copy numbers obtained using different techniques. DNA copy numbers of methylated/unmethylated DNA standards based on specifications of manufacturer (Expected), measured by flourometer and of p14 by Restriction Enzyme (RE) dPCR and p14 and COL2A1 with MethyLight dPCR in the 0% and 100% methylated samples. Copy numbers shown were obtained from 5 ng starting material (based on expected DNA quantity), pre-bisulfite conversion and RE digestion. RE dPCR data shows p14 copy number from the mock, MSRE and MDRE treatments. MethyLight dPCR data shows copy number obtained using the p14_M assay in singleplex and the methylation independent control COL2A1. Statistical comparisons are for Student’s t-test (* = p < 0.05; ns = not significant). Error bars show ± Standard Deviation of measurement.