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. 2014 Dec 23;15(1):1174. doi: 10.1186/1471-2164-15-1174

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© Redshaw et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bisulfite Amplicon NGS. Analysis showing read-based estimates of percent methylation (A-C). (A,B) Correlation between expected and observed percent methylation showing (A) average percent methylation for all samples across both amplicon NGS experiments or (B) all data points plotted individually excluding Multiplex Identifier (MID)9 samples from the analysis in A and B. (C) Correlation between two bisulfite amplicon NGS experiments (including MID9). (D) Correlation between read- and site-based estimates of percent methylation. All correlations were significant at p < 0.0001.