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. Author manuscript; available in PMC: 2015 Aug 3.
Published in final edited form as: Biochemistry. 2015 Mar 26;54(13):2214–2226. doi: 10.1021/acs.biochem.5b00127

Figure 3.

Figure 3

2D 31P-31P exchange spectra of static DMPC/DHPC bicelles. (a) With M2TM-AH and 0.5 s spin diffusion. (b) With M2TM-AH and 1.0 s spin diffusion. (c) Without M2 and with 0.5 s spin diffusion. The spectra were measured at 297 K for (a, b) and 306 K for (c) to obtain the same DHPC 31P chemical shift. Cross peaks between the isotropic peak and the DHPC peak (dotted circle) are observed for the protein-bound bicelles in (a) and (b).