Table 1.
Changes in gene expression in the prospective lens and retina at E10.5.
| Lens Transcripts | |||||
|---|---|---|---|---|---|
| Gene Name | Gene Symbol |
WT | CKO | Fold Change* |
Function in Lens Development |
| Prospero-related homeobox 1 | Prox1 | 976 | 28 | −35 | Required for lens fiber cell formation |
| βB3 crystallin | Crybb3 | 289 | 1 | −289 | Lens-preferred crystallin |
| γE-crystallin | Cryge | 1425 | 60 | −24 | Lens-preferred crystallin |
| γC-crystallin | Crygc | 186 | 1 | −186 | Lens-preferred crystallin |
| γD-crystallin | Crygd | 315 | 11 | −28 | Lens-preferred crystallin |
| Jagged 1 | Jag1 | 1007 | 82 | −12 | Promotes lens epithelial proliferation |
| Musculoaponeuroticfibrosarcoma oncogene | Maf | 1499 | 119 | −13 | Regulates crystallin gene expression |
| Paired-like homeobox-3 | Pitx3 | 153 | 4 | −40 | Lens formation and cell survival |
| N-cadherin | Cdh2 | 228 | 9 | −26 | Lens vesicle separation and survival |
| Forkhead box gene E3 | Foxe3 | 59 | 1 | −59 | Survival of the lens epithelium |
| SRY-box gene 2 | Sox2 | 1204 | 278 | −4 | Interacts with Pax6 for lens formation |
| Retina Transcripts | |||||
|---|---|---|---|---|---|
| Gene Name | Gene Symbol |
WT | CKO | Fold Change* |
Function in Retina Development |
| Aldehyde dehydrogenase family 1 | Aldh1a1 | 2649 | 100 | −26 | Production of retinoic acid |
| Growth and differentiation factor 6 | Gdf6 | 337 | 1 | −337 | Loss causes microphthalmia, coloboma |
| Bone morphogenetic protein 4 | Bmp4 | 311 | 6 | −56 | Retina formation, D-V polarity |
| T-box gene 3 | Tbx3 | 209 | 1 | −209 | Retina D-V patterning |
| Fibroblast growth factor 15 | Fgf15 | 869 | 77 | −11 | Function unknown, highly expressed |
| SRY-box gene 2 | Sox2 | 1528 | 298 | −5 | Maintains retinal progenitor cells |
| Visual system homeobox 2 | Vsx2 | 98 | 4 | −24 | Specifies retina, opposes RPE formation |
| Dickkopf homolog 3 | Dkk3 | 891 | 198 | −5 | Promotes retina gene expression |
| Frizzled 5 | Fzd5 | 109 | 7 | −16 | Wnt receptor, loss causes coloboma |
| Fibroblast growth factor 9 | Fgf9 | 91 | 1 | −91 | Establishes retina-RPE boundary |
| RPE Transcripts | |||||
|---|---|---|---|---|---|
| Gene Name | Gene Symbol |
WT | CKO | Fold Change* |
Function in RPE Development |
| Premelanosome protein | Pmel | 67 | 1460 | 22 | Scaffold for melanosome formation |
| Dopachrometautomerase (Tyrp2) | Dct | 1 | 138 | 138 | Melanin biosynthesis |
| Indolethylamine N-methyltransferase | Inmt | 192 | 1549 | 8 | Melanin biosynthesis |
| Tyrosinase-related protein 1 | Tyrp1 | 4 | 169 | 44 | Melanin biosynthesis |
| Microphthalmia transcription factor | Mitf | 33 | 278 | 8 | Regulation of RPE gene expression |
| Bone morphogenetic protein 7 | Bmp7 | 39 | 295 | 8 | Optic fissure formation and closure |
| RAS oncogene family-38 | Rab38 | 81 | 472 | 6 | Transport of Tyrp1 to the melanosome |
| Stimulated by retinoic acid gene 6 | Stra6 | 32 | 131 | 4 | Retinoic acid uptake |
| Orthodenticle homolog 2 | Otx2 | 472 | 1504 | 3 | Regulation of RPE gene expression |
All comparisons of gene expression in wild type and Bmp4CKO embryos at E10.5 were significantly different at p<0.01.
*
Expression levels for transcripts below 1.0 were rounded up to 1 to calculate estimates of fold change.