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. 2015 Apr 15;21(8):825–831. doi: 10.1089/ten.tec.2014.0500

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Copyright 2015, Mary Ann Liebert, Inc.

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FIG. 3. — (A) Images demonstrating the recovery of differentiation potential after cryopreservation by slow-rate cooling or rapid-cooling vitrification compared to fresh follicles. Red color is for specific antibody staining and blue color is for nuclear staining. (B) Comparison of pluripotency of fresh hair follicles and hair follicles cryopreserved by slow-rate cooling or rapid-cooling vitrification after a one-week culture of hair spheres in DMEM with 10% FBS. Results are mean±SD for three independent experiments (three independent mice for each method of cryopreservation and nonfrozen control).