Fig 7.

FM1-43 uptake of lanceolate endings is modulated by glutamate receptor ligands, indicating it involves the PLD-mGluR. Scatter dot plots of change in FM1-43 intensity as a percentage of drug-free controls. It can be seen that glutamate and kainate both increase, while PCCG-13 decreases intensity, i.e. SLV endocytosis. This is in line with findings in muscle spindles, where they have analogous effects on afferent discharge rates. The effect of LY341495, however, shows that there are differences between these systems, as here it decreases SLV endocytosis whereas it has no effect in muscle spindle firing. Each dot represents a single lanceolate’s FM1-43 intensity as a percentage of the control median. Lines show median intensity of all lanceolates measured, and whiskers indicate the interquartile range of data values (25% and 75%). The width of the distribution is arbitrary, but predominantly reflects the frequency of follicles of that intensity, as the first point is plotted in centrally, and subsequent points increasingly laterally, to minimise overlap and display as many points as possible. For the LY341495 data, many points are seen to overlap, to constrain the lateral spread of the column. Each data set is from 20 to 316 follicles (1 pallisade of lanceolate endings per follicle) per preparation, from 4 to 12 preparations. *P = 0.01, ***P = 0.0001.