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. 2015 May 15;172(15):3748–3763. doi: 10.1111/bph.13162

Figure 4.

Figure 4

Dynasore induces a reduction in monomeric MAVS independently of proteasome degradation. (A) Cells were pretreated with dynasore (80 μM) or DMSO for the times indicated. Cells were extracted and processed for Western blots. For loading correction, nitrocellulose membranes were reprobed with GAPDH. (B) Bands in panel A were subjected to densitometry and expressed as ratio of MAVS/GAPDH. (C) Cells were treated with the indicated concentrations of dynasore for 15 min and processed for Western blots with MAVS, IκBα and GAPDH antibodies. (D) Bands were subjected to densitometry and expressed as ratio of MAVS or IκBα/GAPDH. (E) Cells were pre-incubated with the proteasome inhibitor MG-132 (10 μM) or DMSO for 30 min in HBSS. Cells were then treated with or without the inhibitor plus or minus dynasore (80 μM) or DMSO for 15 min. Cells were lysed and subjected to Western blotting for MAVS, IκBα and reprobed for GAPDH for loading correction. Note that dynasore-induced reduction in MAVS monomer is not inhibited by MG-132, while dynasore-induced degradation of IκBα is inhibited by MG-132.