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. 2015 May 15;172(15):3748–3763. doi: 10.1111/bph.13162

Figure 6.

Figure 6

Knock-down of MAVS with specific siRNA reduces dynasore-induced activation of NF-κB-luciferase. (A) To demonstrate the utility of MAVS siRNA, cells were transfected with 10 nM of control or MAVS siRNA overnight. Cells were subjected to Western blotting analysis using MAVS antibody or β-actin antibody for loading control. (B) Cells were transfected with 10 nM of control or MAVS siRNA overnight. Next day, cells were double- transfected with NF-κB-luciferase and renilla-luciferase for 6 h. Cells were treated with dynasore (80 μM) or DMSO for 15 min in HBSS and then kept in 2% FBS-containing medium overnight. anova: P < 0.002; d.f. within groups = 12.