Skip to main content
. 2015 Jun 12;172(15):3846–3860. doi: 10.1111/bph.13169

Figure 2.

Figure 2

Long-term incubation with agonists or inverse agonists modifies 5-HT7 receptor density. Receptor density (Bmax) in the membranes of HEK293 cells stably expressing the human 5-HT7 receptor splice variants after 24 h of pre-incubation with either 10 μM 5-HT or 1 μM mesulergine, clozapine or SB269970. Radioligand binding was performed with increasing concentrations of [3H]-5-CT in the absence (total binding) and presence (non-specific binding) of 10 μM 5-HT for 1 h at 24°C. Bmax and Kd were determined as described in the Methods section. Data are presented as a % of control (sister plates of cells pre-incubated without drug or vehicle). The data shown are mean ± SEM of 5–14 experiments from two to three different clonal cell lines of each splice variant. *P < 0.01 versus control (one-way anova with Dunnett’s multiple comparison test), P < 0.05 versus the other two 5-HT7 splice variants pre-incubated with the same ligand (two-way anova with Bonferroni adjustment).