Figure 7.

LCB54-0009 inhibits Ang-2 levels and VEGFR-2-signalling cascades. (A–C) HUVECs were incubated for 6 h in hypoxia or 48 h with VEGF (20 ng·mL⁻¹) in the presence or absence of LCB54-0009 (20 μM). The mRNA levels were determined by quantitative real-time PCR (A and B) or reverse transcription PCR (C). Results are expressed as the mean ± SD of three independent experiments (n = 3). #P < 0.05 versus vehicle-treated group; *P < 0.05 versus hypoxia- or VEGF-induced group; ^†P < 0.05 versus LCB54-0009-treated group. (D) HUVECs were incubated with LCB54-0009 for 1 h and then stimulated with VEGF (20 ng·mL⁻¹) for 15 min. Western blotting was performed from the cell lysates. GAPDH served as a loading control.