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Real Time PCR of runt related transcription factor-2 (RUNX2), zinc finger protein Osterix/SP7 and ALP in the cortex and medulla of the control and HLP fed rats. The statistical analyses were performed using GraphPad Prism version 5 for windows (GraphPad software, La Jolla, CA). P-values were calculated using unpaired T-test. P < 0.05 was considered statistically significant. *Control vs. HLP group (P < 0.05), **Control vs. HLP group (P < 0.005), ***Control vs. HLP group (P < 0.0001).
