Table 1.
The list of forward and reverse primers used for quantitative Real Time PCR designed using Primer-BLAST (National Center for Biotechnology Information, NCBI, and National Institute of Health, NIH)
| GENE | PRIMER | SEQUENCE |
|---|---|---|
| Fibronectin (Fn1) | Fn1_F | 5'- GTGGCTGCCTTCAACTTCTC-3' |
| Fn1_R | 5'- GTGGGTTGCAAACCTTCAAT-3' | |
| Runt-related transcription factor-2 (Runx2) | Runx2_F | 5'- TCCCATCTGCTAGAAGTGTT-3' |
| Runx2_R | 5'- TTAGCCAGCTCACTTTCTTC-3' | |
| Osterix/SP7 | Sp7_F | 5'- AAGCCATACACTGACCTTTC-3' |
| Sp7_R | 5'- GTGGGTAGTCATTGGCATAG-3' | |
| Bone Morphogenetic Protein-2 (Bmp2) | Bmp2_F | 5'- ACCAGACTATTGGACACCAG-3' |
| Bmp2_R | 5'- AATCCTCACATGTCTCTTGG-3' | |
| Bone Morphogenetic Protein-7 (Bmp7) | Bmp7_F | 5'- ATGGCCAACGTGGCAGAGAA-3' |
| Bmp7_R | 5'- CAGCCCAGGTCTCGGAAGCT-3' | |
| Bone Morphogenetic Protein Receptor, type II (Bmpr2) | Bmpr2_F | 5'- ATAGGCGTGTGCCAAAAATC-3' |
| Bmpr2_R | 5'- GCTAGGGATTCGAGCTTGTG-3' | |
| Cytokeratin 8 or Keratin 8 (Krt8) | Krt8_F | 5'- AGCCAGAGTACCAGCCCTAA-3' |
| Krt8_R | 5'- ACAATTGAGTTGGGCATTGGC-3' | |
| Cytokeratin 18 or Keratin 18 (Krt18) | Krt18_F | 5'- ATATCCGTGTCCCGCTCTGT-3' |
| Krt18_R | 5'- TCGTTCAGGTCTTGCATGGT-3' | |
| Vimentin (Vim) | Vim_F | 5'- TTCTCAGCACCACGATGACC-3' |
| Vim_R | 5'- TGCTGAGCTCGTTTCTATCCC-3' |