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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: J Proteomics. 2015 Jun 3;126:172–188. doi: 10.1016/j.jprot.2015.05.030

Figure 1. Mass spectrometric analysis of neutral and anionic mosquito N-glycan pools.

Figure 1

Released N-glycans were enriched in neutral and anionic pools prior to pyridylamino-labelling and MALDI-TOF/TOF MS. The spectra shown are those in positive (A, B) and negative ion mode (C, D) of the N-glycan pools released with PNGase F (A, C, D) or PNGase A post PNGase F (B) from Aedes aegypti (A-C) and Anopheles gambiae (D). Abbreviations for annotated [M+H]+ and [M-H]− ions are: H Hexose, N N-acetylhexosamine, F Fucose, S Sulphate and U Glucuronic Acid. The positive ion mode spectra of the neutral Anopheles PNGase F and A pools are not shown due to their similarity to the Aedes; the differences between the two species, limited to the lower intensity glycans, were only resolved after HPLC fractionation.