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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: J Proteomics. 2015 Jun 3;126:172–188. doi: 10.1016/j.jprot.2015.05.030

Figure 4. Elucidation of neutral and anionic triantennary N-glycan structures.

Figure 4

(A) Coelution of Aedes derived triantennary glycans after either defucosylation of the neutral glycan species or solvolysis and defucosylation of the sulphated glycan species with an asialoagalacto-triantennary N-glycan isolated from FCS; note that solvolysis under these conditions is incomplete, but that further incubation resulted in unspecific reaction products. The arrows indicate the shifts in retention time. MALDI TOF/TOF MS spectra in positive (B) and negative ion mode (C-E) of the Aedes anionic triantennary structure eluting at 10.4 g.u. on RP-Amide HPLC show that the anionic triantennary structure is sensitive to β1,2-specific FDL (D) and β1,4-specific ‘chitinase-type’ hexosaminidase (E). MS/MS spectra of parent and product glycan species upon bovine fucosidase digests in positive (F, G) and negative ion mode (H, I) are shown.