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. Author manuscript; available in PMC: 2016 Aug 14.
Published in final edited form as: J Mol Biol. 2015 Jul 6;427(16):2707–2725. doi: 10.1016/j.jmb.2015.06.018

Fig. 4.

Fig. 4

Characterization of target-Fab complexes. (a) aSEC profile of individual target (blue) and target-Fab (green) complex. Fabs form high affinity complexes with target proteins that can be separated by SEC. (b) Melting curves of individual targets (green), Fabs (red) and target-Fab (blue) complex. Fabs are thermally very stable (Tm> 70°C) and stabilize the targets in the complex to an appreciable extent (marked by black arrow). Target-Fab complex was prepared by mixing target: Fab in 1:1.5 molar ratio. The melting peak of the excess Fab in the complex at higher temperature is also observed.