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. Author manuscript; available in PMC: 2016 Aug 14.

Published in final edited form as: J Mol Biol. 2015 Jul 6;427(16):2707–2725. doi: 10.1016/j.jmb.2015.06.018

Fig. 5 — Generation of conformation specific Fabs. (a) Selection strategy to obtain conformation specific Fabs from CBP-tagged targets. CBP-MBP is in open conformation without maltose (green hexagon). In presence of maltose, it adopts the closed conformation. Separate sorting experiments with immobilized targets in specific conformations generate Fabs that are conformation specific. (b) Phage ELISA results show that Fabs generated against closed form of MBP in presence of maltose have a drastically compromised binding to open form of MBP in absence of maltose. (c) SPR sensograms showing that Fabs recognize specific conformation of MBP. Fab 7O binds to open form of MBP immobilized on NTA chip without maltose. No significant binding was observed in 1mM maltose.

Fig. 5 — Generation of conformation specific Fabs. (a) Selection strategy to obtain conformation specific Fabs from CBP-tagged targets. CBP-MBP is in open conformation without maltose (green hexagon). In presence of maltose, it adopts the closed conformation. Separate sorting experiments with immobilized targets in specific conformations generate Fabs that are conformation specific. (b) Phage ELISA results show that Fabs generated against closed form of MBP in presence of maltose have a drastically compromised binding to open form of MBP in absence of maltose. (c) SPR sensograms showing that Fabs recognize specific conformation of MBP. Fab 7O binds to open form of MBP immobilized on NTA chip without maltose. No significant binding was observed in 1mM maltose.