. Author manuscript; available in PMC: 2016 Aug 14.

Published in final edited form as: J Mol Biol. 2015 Jul 6;427(16):2707–2725. doi: 10.1016/j.jmb.2015.06.018

Table 1.

Enrichment of MBP binding phage (MOS1) over control phage in a “doping” selection

Input				Output
Titer MOS1 (cfu/ml)	Titer SETA1 (cfu/ml)	Ratio of titer MOS1: SETA1	Rounds of sorting	Titer MOS1 (cfu/ml)	Enrichment ^a	Ratio^b (MOS1:SETA1)
2.0 X 10⁷	2.0 X 10⁹	1:10²	2^nd	4.0 X 10⁵	10	90:6
			3^rd	3.0 X 10⁶	100	95:1
			4^th	2.0 X 10⁷	>500	96:0
2.0 X 10⁵	2.0 X 10⁹	1:10⁴	2^nd	3.0 X 10⁵	5	79:17
			3^rd	3.0 X 10⁶	30	92:4
			4^th	5.0 X 10⁷	>100	96:0
2.0 X 10²	2.0 X 10⁹	1:10⁷	2^nd	1.0 X 10⁵	-	1:95
			3^rd	2.0 X 10⁶	20	90:6
			4^th	6.0 X 10⁷	>100	96:0

Fold enrichment was calculated based on ratio of titer of MOS1 and SETA1 phage in output.

Ratio of the positive colonies screened by colony PCR in each round. 96 colonies are screened in each round. The number of colonies harboring MOS1 phagemid increases in successive round.