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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Exp Eye Res. 2015 Jun 17;137:84–93. doi: 10.1016/j.exer.2015.06.016

Fig. 1. HIF-1α and HIF-2α are expressed in attached and detached rat retinas.

Fig. 1

(A) Light micrograph of a cryosection from detached retina at 1 day post detachment. (B-C) HIF-1α and HIF-2α protein immunoreactivity on western blots of attached or detached mouse and rat retina protein homogenates, respectively. Samples were collected at 1 and 3 days post detachment. GAPDH served as a loading control. Att, attached retina; D1, 1 day detached retinas; D3, 3 day detached retinas, n=3 animals per time point. (D) HIF-1α and HIF-2α localization on attached and 1 day detached retina cross-sections from C57BL/6J mice with immunohistochemical analysis. Control sections from which primary antibodies are omitted are shown. OS, outer segments; IS, inner segments; ONL, outer nuclear layer.

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