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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Exp Eye Res. 2015 Jun 17;137:84–93. doi: 10.1016/j.exer.2015.06.016

Fig. 2. BNIP3 and LC3 expression in detached rat retinas correlates with HIF-1α and HIF-2α expression.

Fig. 2

(A-B) BNIP3 and LC3 protein immunoreactivity on western blots of attached or detached mouse or rat retina protein homogenates, respectively. Samples were collected at 1 and 3days post detachment. GAPDH served as a loading control. The ratios of LC3-II to LC3-I levels were based on densitometry of respective bands from western blots are shown. Att, attached retina; D1, 1 day detached retinas; D3, 3 day detached retinas. (A) The ratios at 1 and 3 days post-detachment were significantly higher than attached mice retinas (p** < 0.005, p*<0.05, n=3 animals per time point). (B) The ratio at 1 day post-detachment is significantly higher than the attached rat retinas (n=3 animals per time point, p**<0.005, ANOVA followed by Bonferroni’s test). (C) Representative images of BNIP3 immunohistochemical localization on attached and 1 day detached retinas from C57BL/6 mice counter-stained with DAPI. (D) LC3 localization on attached and 1 day detached GFP-LC3 mouse retina cross-sections. Arrows indicate punctate like structures. OS, outer segments; IS, inner segments; ONL, outer nuclear layer.

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