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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jun 11;1852(9):1902–1911. doi: 10.1016/j.bbadis.2015.06.007

Figure 5. Oxygen-glucose deprivation (OGD) produces sex-dependent autophagy in primary cortical neurons from GFP-LC3+/− mice.

Figure 5

The GFP signal was enhanced immunohistochemically. A. Identification of GFP-LC3 enriched vesicles consistent with autophagosome formation at 2 and 24 h after OGD. B. Quantification of GFP-LC3 fluorescent intensity peaks over threshold (fluorescence in control cells) showing that Atg7 siRNA reduces OGD-induced autophagosome formation in XY- and XX-neurons (n = 6/group; *P < 0.05 vs. control siRNA, †P < 0.05 vs. control; ANOVA with Tukey's test). C. GFP immunohistochemistry showing neuronal loss and formation of GFP-enriched vesicles after OGD. D and E. Treatment with Atg7 siRNA reduced LDH release and increased neuron survival detected by PI labeling at 2 and 24 h after OGD in both XY- and XX-neurons vs. control siRNA (LDH, n = 6/group; PI, n = 4/group; *P < 0.05 vs. control siRNA; ANOVA with Tukey's test).