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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Eur J Neurosci. 2015 Jun 19;42(3):1941–1951. doi: 10.1111/ejn.12957

Figure 3.

Figure 3

Normal Htt expression reverses abnormal perinuclear lysosomal positioning in STHdhQ111 cells. A-B. Representative confocal images of Lamp1 staining in (A) STHdhQ7 cells transfected with EGFP alone or fHtt145Q-EGFP and in (B) STHdhQ111 cells transfected with EGFP alone or fHtt23Q-EGFP. Cells were fixed in 2% PFA and stained with Lamp1 (red). C. Top panel, quantitative analysis of perinuclear lysosomal clustering index in STHdhQ7 cells transfected with EGFP (n=21) or Htt145Q-EGFP (n=47) and STHdhQ111 cells transfected with EGFP (n=8) or htt23Q-EGFP (n=29). **P < 0.01, STHdhQ111 vs. STHdhQ7 transfected with EGFP; ***P < 0.001, STHdhQ111 transfected with htt23Q-EGFP vs. STHdhQ111 transfected with EGFP, student's t-test. Bottom panel, western blot analysis of ectopically expressed fHtt23Q-EGFP and fHtt145Q-EGFP in STHdh cells with GFP antibodies.