Fig. 5. Analysis of signaling pathways in 6 mM butyrate-treated LS174T cells.

The synthesized cDNA was applied to a TaqMan array 96-well fast plate, and comparative Ct method for relative quantification was performed. The delta delta Ct value was determined by subtracting the delta Ct of the control sample from the individual delta Ct of the test sample. Significant changes in butyrate-induced gene expression were analyzed based on more than 5-fold changes compared to the control group. MAPKAPK, mitogen-activated protein kinase activated protein kinase; P, protein