Fig. 2A–C.

TAPP stably adsorbs to mineralized bone allograft. (A) Absorbance spectra of TAPP before (125 μM, solid line) and after (TAPP remaining, dashed line) 24-hour incubation at room temperature with bone morsels. (B) Absorbance of solutions that were collected weekly during elution from TAPP-allograft for up to 9 weeks or after exposure to 10 μN (pH 5) or 0.275 N (pH 1) HCl. Values shown are mean ± SD. The graph is a representative experiment with triplicate samples; the experiment was performed a total of three times with similar results. (C) TAPP absorbance was measured in solution (black line). Absorbance of TAPP localized in planktonic S. aureus was measured after incubation (18 hours), washing, and lysis (gray line); and TAPP localized in S. aureus was measured after incubation (18 hours) with TAPP-allograft followed by washing and lysis (broken line).