Photoactive compounds adsorbed to bone allograft combat preformed biofilm. Control, TAPP (cationic), TMPyP (cationic), TSP (anionic), THP (neutral), and methylene blue (MB)-adsorbed allograft were allowed to form S. aureus AH1710 biofilms (48 hours, dark) and bacterial colonization was visualized using the innate green fluorescent protein by confocal laser scanning microscopy after an additional 18 hours in the dark (A–F) or light (G–L). Scale bar = 500 µm.