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The amount of NOG, RUNX-2 and OCN (A) and SP-1 (B) protein was quantified from tissue samples harvest at the site of amputation. (C) The levels of NOG, OCN, and RUNX-2 are statistically significant from the sham-treated (naïve) rats at 3 days postinjury, whereas the levels of SP-1 are significantly different from the sham-treated (naïve) rats at 3 to 7 days postinjury (*p < 0.05; Student’s t-test).
