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. 2015 Aug 3;210(3):451–470. doi: 10.1083/jcb.201501043

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© 2015 Lee et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 9. — Sec22p regulates ER morphology in vivo. (A) The ER was visualized by expressing EGFP-HDEL in wild-type (BY4742) yeast cells. The localization of the fluorescent protein was determined by confocal fluorescence microscopy, with the microscope focused either at the center or periphery of the cell. (B) As in A, but with cells lacking Sey1p (BY4742 sey1Δ). (C) As in A, but with BY4742 rtn1Δ. (D) As in A, but with BY4742 sec22Δ. (E) As in A, but with BY4742 sey1Δ rtn1Δ. (F) As in A, but with BY4742 sec22Δ rtn1Δ. (G) As in A, but with BY4742 sey1Δ sec22Δ. The percentage of cells with abnormal ER was determined from 30–80 cells per strain. Bars, 2 µm.