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. 2015 Aug 3;210(3):451–470. doi: 10.1083/jcb.201501043

Table 1. Yeast strains used in this study.

Strain Genotype Reference
BJ3505 MATa ura3-52 trp1-Δ101 his3- Δ200 lys2-801 gal2 (gal3) can1 prb1-Δ1.6R pep4Δ::HIS3 Jones, 2002
DKY6281 MATα ura3-52 leu2-3,112 trp1-Δ901 his3-Δ200 lys2-801 suc2-Δ9 pho8Δ::TRP1 Haas et al., 1994
BJ-GLuc1 BJ3505 vam3Δ::TRP1 containing pYJ406-ssZIP-GLuc1-HDEL This study
BJ-GLuc2 BJ3505 vam3Δ::TRP1 containing pYJ406-ssZIP-GLuc2-HDEL This study
BJ-GLuc1 sey1Δ BJ-GLuc1 with sey1Δ::KanMX4 This study
BJ-GLuc2 sey1Δ BJ-GLuc2 with sey1Δ::KanMX4 This study
BJ-GLuc1 sec22Δ BJ-GLuc1 with sec22Δ::KanMX4 This study
BJ-GLuc2 sec22Δ BJ-GLuc2 with sec22Δ::KanMX4 This study
BJ-GLuc1 rtn1Δ BJ3505 vam3Δ GLuc1 with rtn1Δ::KanMX4 This study
BJ-GLuc2 rtn1Δ BJ3505 vam3Δ GLuc2 with rtn1Δ::KanMX4 This study
BJ-GLuc1 yop1Δ BJ3505 vam3Δ GLuc1 with yop1Δ::KanMX4 This study
BJ-GLuc2 yop1Δ BJ3505 vam3Δ GLuc2 with yop1Δ::KanMX4 This study
BJ-GLuc1 kar5Δ BJ3505 vam3Δ GLuc1 with kar5Δ::KanMX4 This study
BJ-GLuc2 kar5Δ BJ3505 vam3Δ GLuc2 with kar5Δ::KanMX4 This study
BJ-GLuc1 sec22Δ SEY1-OE BJ-GLuc1 sec22Δ containing pRS408-pTDH3-SEY1 This study
BJ-GLuc2 sec22Δ SEY1-OE BJ-GLuc2 sec22Δ containing pRS408-pTDH3-SEY1 This study
BJ-GLuc1 Sey1-K50A BJ-GLuc1 sey1Δ containing pRS408-SEY1-K50A This study
BJ-GLuc2 Sey1-K50A BJ-GLuc2 sey1Δ containing pRS408-SEY1-K50A This study
RSY250 MATα ura3-52 Kaiser and Schekman, 1990
RSY275 MATα ura3-52 his4-619 s20-1 Kaiser and Schekman, 1990
RSY250 GLuc1 RSY250 containing pYJ406-ssZIP-GLuc1-HDEL This study
RSY250 GLuc2 RSY250 containing pYJ406-ssZIP-GLuc2-HDEL This study
RSY275 GLuc1 RSY275 containing pYJ406-ssZIP-GLuc1-HDEL This study
RSY275 GLuc2 RSY275 containing pYJ406-ssZIP-GLuc2-HDEL This study
BY4742 MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 Winzeler et al., 1999
BY-EGFP-HDEL BY4742 with pYJ406-ssEGFP-HDEL This study
BY-EGFP-HDEL sey1Δ BY4742 EGFP-HDEL with sey1Δ::KanMX4 This study
BY-EGFP-HDEL rtn1Δ BY4742 EGFP-HDEL with rtn1Δ::KanMX4 This study
BY-EGFP-HDEL sec22Δ BY4742 EGFP-HDEL with sec22Δ::KanMX4 This study
BY-EGFP-HDEL sey1Δ rtn1Δ BY4742 rtn1Δ EGFP-HDEL with sey1Δ::LEU2 This study
BY-EGFP-HDEL sec22Δ rtn1Δ BY4742 sec22Δ EGFP-HDEL with rtn1Δ::LEU2 This study
BY-EGFP-HDEL sec22Δ sey1Δ BY4742 sec22Δ EGFP-HDEL with sey1Δ::LEU2 This study