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. 2015 Aug 3;210(3):401–417. doi: 10.1083/jcb.201506110

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© 2015 Ganguly et al.

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Figure 1. — F-actin dynamics in axons. Neurons were transfected with GFP:Utr-CH to label F-actin (and soluble mRFP to visualize morphology), and F-actin dynamics in axons were imaged as described in methods. (A) Selected frames from a representative video showing anterograde (left) and retrograde (right) dynamics of actin polymers (dynamic tips marked by single/double arrowheads). Image far below shows soluble mRFP in the same axon. Elapsed time in seconds on left/right (see Video 1). (B and C) Kymograph of the video in A with first frame on top shown in B. Boxed ROIs are zoomed in C. Note unusual F-actin dynamics including diagonal, vectorial plumes of fluorescence (actin trails) and hotspots (vertical interrupted lines). Yellow and red arrowheads represent the same dynamic structures in A and B. Elapsed time is given in seconds on the left. (D) Cropped kymographs from other videos show focal F-actin hotspots (some marked by asterisks). Instances of actin trails emerging from—and collapsing into—hotspots can also be seen. Note trails marked by arrows 1, 2, 6, and 7 are emerging from hotspots. Antero, anterograde; Retro, retrograde.