Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 3;210(3):419–433. doi: 10.1083/jcb.201411037

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Matsubayashi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Dynamin-dependent relocalization of Dia during wound healing. (A, top) Time-lapse live imaging of embryos expressing GFP-Dia or GFP together with mCherry-Moesin in wild-type embryos. Note that GFP-Dia is recruited to wound edge junctions after wounding (arrowheads), whereas GFP alone is not (rightmost image). (bottom) Behavior of GFP-Dia in a wounded shi² embryo. Note that the fluorescence level at wound edge junctions does not increase (arrowheads). Time points indicate time after wounding (minutes and seconds). (B) The enlarged images of GFP-Dia (green) and mCherry-Moesin (magenta) in the area indicated by the dashed square in A (Control, 8:20). Note the colocalization of GFP-Dia and actin puncta (arrowheads). (C and D) Dia accumulation over the course of wound healing in wild-type and shi² embryos, measured in two distinct ways as described in Materials and methods. (C) Semiquantification based on the size and brightness of GFP-Dia accumulation at wound edge junctions. n = 5–7 embryos. Each image contained 3–23 wound edge junctions. (D) Quantification of the GFP-Dia fluorescence intensity at wound edge junctions. n = 27–31. Note that in both C and D, the value indicating the accumulation of GFP-Dia increases after wounding in control but not shi² embryos. All experiments were performed at 30°C. Graphs show means ± SEM of the data. Bars, 10 µm.