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. 2015 Aug 3;210(3):485–502. doi: 10.1083/jcb.201501038

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© 2015 Cheng et al.

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Figure 3. — MTM-1, OCRL-1, PIKI-1, and LST-4 are required for phagosomal sealing. (A) Cartoon illustrating the examination of phagosomal sealing by FM4-64 staining. An unrecognized cell corpse (left) or unsealed phagosome (middle) can be stained by the membrane-impermeable dye FM4-64 (red), whereas a fully separated phagosome (right) is not accessible to FM4-64. (B–K’’) DIC and fluorescent images of the gonads in the indicated strains expressing GFP::PLCδ1-PH and stained by FM4-64. Germ cell corpses are boxed, and arrowheads point to one cell corpse that is labeled by both FM4-64 and GFP::PLCδ1-PH. (L and M) The percentage of cell corpses labeled by FM4-64 (L) and GFP::PLCδ1-PH (M) was quantified in the indicated strains. At least 10 animals were scored in each strain. Data are shown as mean ± SD. The gla-3 RNAi dataset was compared with all the other datasets by a one-way ANOVA with Tukey’s post-test. PM, plasma membrane. *, P < 0.05; **, P < 0.0001. Bars, 10 µm.