LST-4 acts at the PtdIns(4,5)P2-positive stage, and its dynamics are regulated by PtdIns(4,5)P2, PIKI-1, and MTM-1. (A) Time-lapse images of a cell corpse in a wild-type embryo coexpressing mCHERRY::MTM-1(C378S) and LST-4::GFP. (B and C) Time-lapse images of germ cell corpses in a gla-3RNAi (B) or an ocrl-1RNAi (C) animal coexpressing LST-4::mCHERRY and YFP::2xFYVE. (D) Time-lapse images of germ cell corpses in the indicated strains coexpressing GFP::ACT-1 and LST-4::mCHERRY. GFP::ACT-1 indicates internalization of apoptotic cells. (C and D) The percentage of phagosomes with the representative pattern is shown at the right. “n” indicates the number of phagosomes that were followed and quantified. Arrowheads in C point to the LST-4::mCHERRY-positive phagosome in the ocrl-1RNAi animal. (A–D) Images in 20–25 z series were captured. Representative images are shown. (E) Germ cell corpses were quantified in the indicated strains at 24 and 48 h after L4/adult molt. At least 15 animals were scored in each strain at each stage. Data are shown as mean ± SD. A two-way ANOVA was performed. No significant differences were found (genotype variation, P = 0.7157). Bars, 5 µm.